Research Abstract

Dr. Fitzpatrick’s primary research interests lie in the integration and application of multi-scale optical and charged particle imaging technologies -- specifically, biological applications of ion microscopy, development of correlative 3D light and electron microscopy approaches, and new computational tools to visualize and manipulate large-scale multi-dimensional datasets.

Selected Publications

  • A.J. Calhoun, A. Tong, N. Pokala, J.A.J Fitzpatrick, T.O. Sharpee and S.H. Chalasani. 2015. Neural Mechanisms for evaluating environemtal variability in Caenorhabditis elegans. Neuron. 86, 1-14, April 22, 2015
  • M.W. Adams, A.F. Loftus, S.E. Dunn, M.S. Joens and J.A.J. Fitzpatrick. 2015. Invited Chapter on "Light-Sheet Fluorescence Microscopy". Current Protocols in Cytometry. 71:12.37.1-12.37.15.
  • H. Contreras, M.S. Joens, L.M. McMath, V.P. Le, M.V. Tullius, J.M. Kimmey, N. Bionghi, M.A. Horwitz, J.A.J. Fitzpatrick and C.W. Goulding. 2014. Characterization of a Mycobacterium tuberculosis nanocompartment and its potential cargo proteins. Journal of Biological Chemistry\., May 22 pii: jbc.M114.570119.
  • M.S. Joens, C. Huynh, J.M. Kasuboski, D. Ferranti, Y.J. Sigal, F. Zeitvogel, M. Obst, C.J. Burkhardt, K.P. Curran, S.H. Chalasani, L.A. Stern, B. Goetze and J.A.J. Fitzpatrick. 2013. Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution. Scientific Reports. 3, 3514.
  • Y.S. Hu, Q. Zhu, K. Elkins, K. Tse, Y. Li, J.A.J. Fitzpatrick, I.M. Verma and H. Cang. 2013. Light-sheet Bayesian microscopy enables deep-cell super-resolution imaging of heterochromatin in live human embryonic stem cells. Optical Nanoscopy. 2:7.
  • Y.H. Chien, M.E. Werner, J. Stubbs, M.S. Joens, J. Li, S. Chien, J.A.J. Fitzpatrick, B.J. Mitchell and C. Kintner. 2013. Bbof1 is required to maintain cilia orientation. Development. Aug;140(16):3468-77.
  • L. Crabbe, A.J. Cesare, J.M. Kasuboski, J.A.J. Fitzpatrick, and J. Karlseder. 2012. Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly. Cell Reports. Dec 27;2(6):1521-9.
  • H. D. Ou, W Kwiatkowski, T Deerinck, A Noske, K.Y. Blain, H Land, C Soria, C.J. Powers, X Shu, R Tsien, J.A.J. Fitzpatrick, J.A. Long, M.H. Ellisman, S Choe and C.C. O'Shea. 2012. Structure of E4-ORF3 reveals a viral supramolecular assembly that inactivates multiple tumor suppressors. Cell. Oct 12; 151(2):304-19.
  • J.M. Kasuboski, Y.J. Sigal, M.S. Joens, B.F. Lillemeier and J.A.J. Fitzpatrick. 2012. Invited Chapter on "Super-Resolution Microscopy: a comparative treatment" Current Protocols in Cytometry. Oct; Chapter 2: Unit 2.17.
  • M. Hatori, C. Vollmers, A. Zarrinpar, L. Ditacchio, E.A. Bushong, S. Gill, M. Leblanc, A. Chaix, M.S. Joens, J.A.J. Fitzpatrick, M.H. Ellisman and S. Panda. 2012. Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet. Cell Metabolism. Jun 6; 15(6):848-60.
  • S. Shruti, J. Urban-Ciecko, J.A.J. Fitzpatrick, R. Brenner, M.P. Bruches and A.L. Barth. 2012. The Brain- Specific Beta4 Subunit Downregulates BK Channel Cell Surface Expression. PLoS One. 7(3) e33429.
  • M.T. Hayashi, A.J. Cesare, J.A.J. Fitzpatrick, E. Lazzerini-Denchi and J. Karlseder. 2012. A Telomere-Dependent DNA Damage Checkpoint induced by Prolonged Mitotic Arrest. Nature Structural and Molecular Biology. 11(19) 387-94.
  • J.A.J. Fitzpatrick and B.L. Lillemeier. 2011. Fluorescence Correlation Spectroscopy: Linking Molecular Dynamics to Biological Function in vitro and in situ. Current Opinion in Structural Biology. 21(5) 650-60.
  • D.F. Egan, D.B. Shackelford, M.M. Mihaylova, S.R. Gelino, R.A. Kohnz, W Mair, D.S. Vasquez, A Joshi, D.M. Gwinn, R Taylor, J.M. Asara, J.A.J. Fitzpatrick, A Dillin, B Viollet, M Kundu, M Hansen, and R.J. Shaw. 2011. Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase connects energy sensing to mitophagy. Science. 331(6016) 456-461.
  • K. Padmanabhan, Shane E. Andrews and J.A.J. Fitzpatrick. 2010. Invited Chapter on "Mutli-Photon Imaging". Current Protocols in Cytometry. 2.9.1-2.9.12.
  • C.S. Szent-Gyorgyi, B.F. Schmidt, J.A.J. Fitzpatrick and M.P. Bruchez. 2010. Fluorogenic dendrons with multiple donor chromophores as bright genetically targeted and activated probes. Journal of the American Chemical Society. 132(32):11103-9.
  • E. Fishilevich, J.A.J. Fitzpatrick and J.S. Minden. 2010. pHMA, a pH-sensitive GFP reporter for cell engulfment, in Drosophila embryos, tissues, and cells. Developmental Dynamics. 239(2), 559-573.
  • S. Shenoy, R. Moldovan, J.A.J. Fitzpatrick, M. Deserno and M. Lösche. 2010. In-plane homogeneity and lipid dynamics in tethered bilayer lipid membranes (tBLMs). Softmatter. 6,1263-1274.
  • J.A.J. Fitzpatrick, S.K. Andreko, L.A. Ernst, A.S. Waggoner, B. Ballou and M.P. Bruchez. 2009. Long-term persistence and spectral blue shifting of quantum dots in vivo. Nano Letters. 9(7), 2736–2741. Highlighted in Chemical and Engineering News, June 2009.
  • J.A.J. Fitzpatrick, Q. Yan, J.J. Sieber, M. Dyba, C. Szent-Gyorgyi, A.S. Waggoner and M.P. Bruchez. 2009. STED nanoscopy in living cells using fluorogen activating proteins. Bioconjugate Chemistry. 20(10), 1843-1847.
  • M.M. Witzberger, J.A.J. Fitzpatrick, J.C. Crowley and J.S. Minden. 2008. End-on Imaging: A new perspective on dorsoventral development in drosophila embryos. Developmental Dynamics, 237(11), 3252-3259, PMID: 18855896.
  • C. Szent-Gyorgyi, B.F. Schmidt, Y. Creeger, G. Fisher, K. Zakel, S. Adler, J.A.J. Fitzpatrick, C. Woolford, Q. Yan, K. Vasilev, P. Berget, M.P. Bruchez, J. Jarvik, A.S. Waggoner. 2008. Fluorogen-activating single-chain antibodies for imaging cell surface proteins. Nature Biotechnology. 26(2), 235-249, PMID: 18157118. Featured article in issue.
  • S.K. Chakraborty, J.A.J. Fitzpatrick, J.A. Phillippi, S. Andreko, M.P. Bruchez and B. Ballou. 2007. Choleratoxin B conjugated quantum dots for live cell labeling. Nano Letters. 7(9), 2618-2626, PMID: 17663586. Highlighted in Nature Nanotechnology Sept 2008.

James Fitzpatrick, PhD

Scientific Director, Washington University Center for Cellular Imaging
Professor of Neuroscience and Cell Biology & Physiology

Washington University
School of Medicine
Campus Box 8108
St. Louis, MO 63110
(314) 747-0838


Fitzpatrick Lab

Other Information

1997-2000, B.Sc. in Chemistry, King's College London, U.K.

2000-2003, PhD in Optical Physics, Laser Spectroscopy and Dynamics Group, University of Bristol, U.K

2003-2004, NSF Postdoctoral Fellow, University of Pittsburgh, PA, USA

2004-2006,NIH Postdoctoral Fellow, Carnegie Mellon University, PA USA

Selected Honors
2013 Editor, ChemPhysChem Special Issue – Super-resolution Imaging and Nanophotonics ()

2011-present Associate Review Editor, Frontiers in Genomic Assay Technology

2009 Long-term quantum dot research featured in Chemical & Engineering News

2008 Cholera-toxin-B quantum dot research featured in Nature Nanotechnology

2008 FAP imaging technology featured in Nature Biotechnology

2002 Institute of Physics Research Travel Award

2001 and 2002 ICI "Excellence in Graduate Research" prize University of Bristol